BIOCONTROL POTENTIAL OF BACILLUS THURINGIENSIS ISOLATED FROM SOIL SAMPLES AGAINST LARVA OF MOSQUITO

ABSTRACT

A major challenge for achieving successful mosquito control is overcoming insecticide resistance. Bacillus thuringiensis which is one of the most effective biolarvacide for control of species of mosquitoes and monitoring of larval susceptibility is essential to avoid resistance development. Mosquito larvacidal activity of Bacillus thuringiensis was assessed by isolating them from ecologically different soil habitats in and around Enugu metropolis. The isolate organisms were confirmed as Bacillus thuringiensis based on biochemical characterization and microscopic observation. The larvacidal activity of Bacillus thuringiensis isolates was tested against the larval of mosquito by using the standard cup bioassay. The isolates of Bacillus thuringiensis showed a significant level of variation in their larvacidal activity.

CHAPTER ONE

INTRODUCTION

Bacillus thuringrensis (Bt) is a well known and widely studied bacterium which is known for its use in pest management. Today it is the most successful commercial xenobiotic with its worldwide application when compared with the chemical pesticides; Bacillus thuringiensis has the advantages of being biologically degradable, selectively active on pests and less likely to cause resistance. Safety of Bacillus thuringiensis formulations for humans, beneficial animals and plants explains the replacement of chemical pesticides in many countries with these environmentally friendly pest control agents.

Bacillus thuringiensis was first isolated by the Japanese Scientist Ishiwata (1901) from skilkworm larvae, bombyxmori, exhibiting sotto disease. After 10 years, Berliner (1911) isolated the square gram (+) positive, spore-forming, rod shaped soil bacterium from disease flour moth larvae, Anngasta Kachmiccalla, in the Thuringia region of the Germany and named it as

Bacillus thuringiensis.

In the early 1930s Bacillus thuringiensis was used against Ostrinianubilis, the European corn borer. The first commercial product was available in 1938 in France, with the trade name sporeine (Weiser, 1986). It was Bacillus thuringiensis subspecies Kurstaki that was used for the control of the insect.....

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Item Type: Project Material  |  Size: 43 pages  |  Chapters: 1-5

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